Plasmid

Part:BBa_K209399:Design

Designed by: Oliver Hoeller   Group: iGEM09_UCSF   (2009-09-14)

AarI A-D acceptor vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 116


Design Notes

carries Gateway sites outside the A-D ends, so that an assembled device can be shuttled into any Gateway destination vector; 'A' starts at nt 673, 'D' ends at nt 754;

Source

modified pDONR221

References